A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology¿ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
| Serija: | Methods in Molecular Biology |
| Leidėjas: | Humana Press |
| Išleidimo metai: | 2005 |
| Knygos puslapių skaičius: | 584 |
| ISBN-10: | 1588293726 |
| ISBN-13: | 9781588293725 |
| Formatas: | 241 x 160 x 41 mm. Knyga kietu viršeliu |
| Kalba: | Anglų |
Parašykite atsiliepimą apie „ProteinLigand Interactions: Methods and Applications“
