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We present a comparative study of monoterpenes in in vitro and field grown plants of Mentha arvensis L. (Japanese mint). Apical and nodal explants were cultured on MS agar-solidified media + BAP (0.2 to 1 mgL-1) for shoot induction. Highest shoot induction was obtained at 1mgL-1 BAP from both explants. The number of shoots was highest at 0.75 mgL-1 Kinetin and 1mgL-1 BAP from axenic shoots. In vitro grown shoots were cultured in ¿ MS salts + IBA (2 mgL-1) for rooting. Excellent callus was achieved from leaf explants cultured on MS medium + 2,4-D 3 mgL-1 or 0.25 mgL-1. In vitro, field grown and callus were subjected for extraction and evaluation of monoterpenes. There were 17 constituents detected from field grown plants by Gas Chromatography- Mass Spectrometery (GC-MS) of which menthol was the major component (57.44%) as compared to menthone (9.17%) and pulegone (7.73%), whereas, menthone with 65.32% was the main component of oil extracted from 16 weeks old calli followed by menthol (0.11%) and pulegone (0.21%). Mint oil extracted from in vitro plants showed variable concentration of 15 different compounds .
| Autorius: | Muhammad Akram, Faheem Aftab, |
| Leidėjas: | LAP LAMBERT Academic Publishing |
| Išleidimo metai: | 2020 |
| Knygos puslapių skaičius: | 56 |
| ISBN-10: | 6200529922 |
| ISBN-13: | 9786200529923 |
| Formatas: | 220 x 150 x 4 mm. Knyga minkštu viršeliu |
| Kalba: | Anglų |
Parašykite atsiliepimą apie „Monoterpene contents in Mentha arvensis L.: GC-MS analysis of callus, in vitro and field-grown shoots of Japanese mint“
