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Double electron electron resonance (DEER) is an EPR technique which allows for distance measurements on the nanometer scale. Here, EPR-based distance measurements were extended to in-cell applications. From evaluation of nitroxides for experiments in intracellular environment of Xenopus laevis oocytes and through proof-of-principle experiments optimal parameters for performing in-cell DEER were found. On the example of G-quadruplexes formed by oligomers of human telomeric DNA, DEER was shown to be applicable for structural identification of biological objects under their native conditions.
| Autorius: | Mykhailo Azarkh |
| Leidėjas: | Südwestdeutscher Verlag für Hochschulschriften AG Co. KG |
| Išleidimo metai: | 2015 |
| Knygos puslapių skaičius: | 152 |
| ISBN-10: | 3838134400 |
| ISBN-13: | 9783838134406 |
| Formatas: | 220 x 150 x 10 mm. Knyga minkštu viršeliu |
| Kalba: | Anglų |
Parašykite atsiliepimą apie „In-cell approaches in electron paramagnetic resonance spectroscopy: In-cell approaches in electron paramagnetic resonance spectroscopy to study conformations of DNA G-quadruplexes“
