The first chapter is a summary of recent publications about the effect of HNO in the cardiovascular system, detection of HNO in vitro, and physiological reactivity of HNO. The second chapter provides synthetic details of a newly developed method of a synthesis of HNO-Mb of high purity (> 95%). The transient absorbance studies of HNO-Mb revealed completely different results than that of other myoglobin adducts such as MbO2 and NO- Mb. Formation of the Mb-Fe(III)/HNO- geminate pair was confirmed by analyses of transient absorbance spectra at 395 nm. In the third chapter rate constants of the reaction of HNO with Mb-Fe(II), Mb- Fe(III), HNO-Mb, and HNO-Mb with oxygen and nitric oxide were determined using the computational software React for Windows. In addition to the computational method, a direct method of comparison of the rates of the reaction of HNO with proteins was developed. In the last chapter synthesis of HNO adduct of hemoglobin is reported. UV-vis, EPR, and 1H NMR spectroscopic techniques were used to identify and differentiate the hydride peaks of HNO subunits of hemoglobin.
| Autorius: | Dmitry Pervitsky |
| Leidėjas: | LAP LAMBERT Academic Publishing |
| Išleidimo metai: | 2009 |
| Knygos puslapių skaičius: | 108 |
| ISBN-10: | 3838301587 |
| ISBN-13: | 9783838301587 |
| Formatas: | 220 x 150 x 7 mm. Knyga minkštu viršeliu |
| Kalba: | Anglų |
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