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CRISPR-Cas Methods

-15% su kodu: ENG15
168,28 
Įprasta kaina: 197,98 
-15% su kodu: ENG15
Kupono kodas: ENG15
Akcija baigiasi: 2025-03-03
-15% su kodu: ENG15
168,28 
Įprasta kaina: 197,98 
-15% su kodu: ENG15
Kupono kodas: ENG15
Akcija baigiasi: 2025-03-03
-15% su kodu: ENG15
2025-02-28 197.9800 InStock
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Knygos aprašymas

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Informacija

Serija: Springer Protocols Handbooks
Leidėjas: Springer New York
Išleidimo metai: 2020
Knygos puslapių skaičius: 296
ISBN-10: 1071606182
ISBN-13: 9781071606186
Formatas: 254 x 178 x 17 mm. Knyga minkštu viršeliu
Kalba: Anglų

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