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In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.
| Autorius: | Jiaqian Wu |
| Leidėjas: | LAP LAMBERT Academic Publishing |
| Išleidimo metai: | 2011 |
| Knygos puslapių skaičius: | 204 |
| ISBN-10: | 3844320601 |
| ISBN-13: | 9783844320602 |
| Formatas: | 220 x 150 x 13 mm. Knyga minkštu viršeliu |
| Kalba: | Anglų |
Parašykite atsiliepimą apie „CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY: FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY“
