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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

-15% su kodu: ENG15
143,97 
Įprasta kaina: 169,38 
-15% su kodu: ENG15
Kupono kodas: ENG15
Akcija baigiasi: 2025-03-03
-15% su kodu: ENG15
143,97 
Įprasta kaina: 169,38 
-15% su kodu: ENG15
Kupono kodas: ENG15
Akcija baigiasi: 2025-03-03
-15% su kodu: ENG15
2025-02-28 169.3800 InStock
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Knygos aprašymas

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer¿s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

Informacija

Autorius: Fangjun Wang
Serija: Springer Theses
Leidėjas: Springer Berlin Heidelberg
Išleidimo metai: 2013
Knygos puslapių skaičius: 108
ISBN-10: 3642420079
ISBN-13: 9783642420078
Formatas: 241 x 160 x 12 mm. Knyga kietu viršeliu
Kalba: Anglų

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